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mirvana mirna bioarrays v.2  (Thermo Fisher)


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    Thermo Fisher mirvana mirna bioarrays v.2
    Mirvana Mirna Bioarrays V.2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mirna bioarrays v.2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mirvana mirna bioarrays v.2 - by Bioz Stars, 2026-04
    90/100 stars

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    mirvana mirna bioarrays v.2  (Thermo Fisher)
    90
    Thermo Fisher mirvana mirna bioarrays v.2
    Mirvana Mirna Bioarrays V.2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mirna bioarrays v.2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mirvana mirna bioarrays v.2 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    mirvana mirna bioarrays v. 2  (Thermo Fisher)
    90
    Thermo Fisher mirvana mirna bioarrays v. 2
    A) <t>miRNA</t> microarray profiles were performed on RNA extracted from HT-29 cells treated with vehicle or 100 μM ACh for 4 h. Data were normalized using the global Lowess regression algorithm and are expressed as log base 2 transformed ratios of the sample signal to the control reference pool signal. miRNAs with statistically significant changes (p<0.05) after ACh treatment are shown in the heat map; red represents increased expression compared to the pooled reference control, and green represents decreased expression. B) Changes in miR-21, miR-221, and miR-222 expression were confirmed by qPCR in HT-29 and H508 human colon cancer cells. Results are means ± SE, n = 4. *, indicates p<0.05 for the test sample compared to control. C) Expression of miR-21, miR-221, and miR-222 in sporadic colonic cancers and adjacent normal-appearing tissue were measured by microarray. Results are means ± SE, n = 4. *, indicates p<0.05 for expression in cancer compared to normal.
    Mirvana Mirna Bioarrays V. 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana mirna bioarrays v. 2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mirvana mirna bioarrays v. 2 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    mirvana bioarray v.2  (Thermo Fisher)
    90
    Thermo Fisher mirvana bioarray v.2
    A) <t>miRNA</t> microarray profiles were performed on RNA extracted from HT-29 cells treated with vehicle or 100 μM ACh for 4 h. Data were normalized using the global Lowess regression algorithm and are expressed as log base 2 transformed ratios of the sample signal to the control reference pool signal. miRNAs with statistically significant changes (p<0.05) after ACh treatment are shown in the heat map; red represents increased expression compared to the pooled reference control, and green represents decreased expression. B) Changes in miR-21, miR-221, and miR-222 expression were confirmed by qPCR in HT-29 and H508 human colon cancer cells. Results are means ± SE, n = 4. *, indicates p<0.05 for the test sample compared to control. C) Expression of miR-21, miR-221, and miR-222 in sporadic colonic cancers and adjacent normal-appearing tissue were measured by microarray. Results are means ± SE, n = 4. *, indicates p<0.05 for expression in cancer compared to normal.
    Mirvana Bioarray V.2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirvana bioarray v.2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mirvana bioarray v.2 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    A) miRNA microarray profiles were performed on RNA extracted from HT-29 cells treated with vehicle or 100 μM ACh for 4 h. Data were normalized using the global Lowess regression algorithm and are expressed as log base 2 transformed ratios of the sample signal to the control reference pool signal. miRNAs with statistically significant changes (p<0.05) after ACh treatment are shown in the heat map; red represents increased expression compared to the pooled reference control, and green represents decreased expression. B) Changes in miR-21, miR-221, and miR-222 expression were confirmed by qPCR in HT-29 and H508 human colon cancer cells. Results are means ± SE, n = 4. *, indicates p<0.05 for the test sample compared to control. C) Expression of miR-21, miR-221, and miR-222 in sporadic colonic cancers and adjacent normal-appearing tissue were measured by microarray. Results are means ± SE, n = 4. *, indicates p<0.05 for expression in cancer compared to normal.

    Journal: PLoS ONE

    Article Title: Muscarinic receptor activation in colon cancer selectively augments pro-proliferative microRNA-21, microRNA-221 and microRNA-222 expression

    doi: 10.1371/journal.pone.0269618

    Figure Lengend Snippet: A) miRNA microarray profiles were performed on RNA extracted from HT-29 cells treated with vehicle or 100 μM ACh for 4 h. Data were normalized using the global Lowess regression algorithm and are expressed as log base 2 transformed ratios of the sample signal to the control reference pool signal. miRNAs with statistically significant changes (p<0.05) after ACh treatment are shown in the heat map; red represents increased expression compared to the pooled reference control, and green represents decreased expression. B) Changes in miR-21, miR-221, and miR-222 expression were confirmed by qPCR in HT-29 and H508 human colon cancer cells. Results are means ± SE, n = 4. *, indicates p<0.05 for the test sample compared to control. C) Expression of miR-21, miR-221, and miR-222 in sporadic colonic cancers and adjacent normal-appearing tissue were measured by microarray. Results are means ± SE, n = 4. *, indicates p<0.05 for expression in cancer compared to normal.

    Article Snippet: Human miRNA profiles were analyzed using Exiqon miRNA array 7th Gen (Product #208500) for HT-29 cells and mirVana miRNA Bioarrays v. 2 (Thermo Fisher Scientific) for human colon samples according to the manufacturer’s instructions.

    Techniques: Microarray, Transformation Assay, Control, Expressing

    A) Pri-miR-21 and B) pri-miR-221/222 levels were measured by qPCR in HT-29 cells. Cells were pre-incubated for 45 min with 5 μM atropine to inhibit M 3 R activation, 5 μM Gӧ6976 to inhibit PKC activation, and 10 μM SB203580 (SB203) to inhibit p38 activation. Then, 100 μM ACh was added for an additional 60-min incubation. Bars represent means ± SEM. n = 4. *, indicates p< 0.05 compared to 100 μM ACh alone. C) HT-29 cells were pre-incubated for 45 min with 5 μM atropine before adding 100 μM ACh or transfecting cells with miR-222 mimics for an additional 24 h. Cell proliferation was measured using the WST-1 assay. Bars represent means ± SEM. n = 4 *, P < 0.05 compared to cells without atropine. D) Working model for post-M 3 R signal transduction mediating muscarinic receptor agonist-induced increases in selected miRNAs in colon cancer. The agonists, inhibitors, and miRNA mimics used to test each step in the proposed signaling pathway are shown.

    Journal: PLoS ONE

    Article Title: Muscarinic receptor activation in colon cancer selectively augments pro-proliferative microRNA-21, microRNA-221 and microRNA-222 expression

    doi: 10.1371/journal.pone.0269618

    Figure Lengend Snippet: A) Pri-miR-21 and B) pri-miR-221/222 levels were measured by qPCR in HT-29 cells. Cells were pre-incubated for 45 min with 5 μM atropine to inhibit M 3 R activation, 5 μM Gӧ6976 to inhibit PKC activation, and 10 μM SB203580 (SB203) to inhibit p38 activation. Then, 100 μM ACh was added for an additional 60-min incubation. Bars represent means ± SEM. n = 4. *, indicates p< 0.05 compared to 100 μM ACh alone. C) HT-29 cells were pre-incubated for 45 min with 5 μM atropine before adding 100 μM ACh or transfecting cells with miR-222 mimics for an additional 24 h. Cell proliferation was measured using the WST-1 assay. Bars represent means ± SEM. n = 4 *, P < 0.05 compared to cells without atropine. D) Working model for post-M 3 R signal transduction mediating muscarinic receptor agonist-induced increases in selected miRNAs in colon cancer. The agonists, inhibitors, and miRNA mimics used to test each step in the proposed signaling pathway are shown.

    Article Snippet: Human miRNA profiles were analyzed using Exiqon miRNA array 7th Gen (Product #208500) for HT-29 cells and mirVana miRNA Bioarrays v. 2 (Thermo Fisher Scientific) for human colon samples according to the manufacturer’s instructions.

    Techniques: Incubation, Activation Assay, WST-1 Assay, Transduction